|
|
|
| Registros recuperados: 21 | |
|
|
| Lima,Jamily L.R. de; Soares,Fabricio A.; Remedios,Ana C.S.; Thom,Gregory; Wirthlin,Morgan; Aleixo,Alexandre; Schneider,Maria Paula C.; Mello,Claudio V.; Schneider,Patricia N.. |
| The memorization and production of song in songbirds share important parallels with the process of speech acquisition in humans. In songbirds, these processes are dependent on a group of specialized telencephalic nuclei known as the song system: HVC (used as a proper name), RA (robust nucleus of arcopallium), LMAN (lateral magnocellular nucleus of the nidopallium) and striatal Area X. A recent study suggested that the arcopallium of the Sayornis phoebe, a non vocal learner suboscine species, contains a nucleus with some properties similar to those of songbird RA, suggesting that the song system may have been present in the last common ancestor of these groups. Here we report morphological and gene expression evidence that a region with some properties... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vocal learning; RGS4; Song nuclei; In situ hybridization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300249 |
| |
|
| |
|
|
| Omoregie, E; Mastalerz, V; De Lange, G; Straub, K; Kappler, A; Roy, H; Stadnitskaia, A; Foucher, Jean-paul; Boetius, A. |
| In this study we determined the composition and biogeochemistry of novel, brightly colored, white and orange microbial mats at the surface of a brine seep at the outer rim of the Chefren mud volcano. These mats were interspersed with one another, but their underlying sediment biogeochemistries differed considerably. Microscopy revealed that the white mats were granules composed of elemental S filaments, similar to those produced by the sulfide-oxidizing epsilonproteobacterium "Candidatus Arcobacter sulfidicus." Fluorescence in situ hybridization indicated that microorganisms targeted by a "Ca. Arcobacter sulfidicus"-specific oligonucleotide probe constituted up to 24% of the total the cells within these mats. Several 16S rRNA gene sequences from organisms... |
| Tipo: Text |
Palavras-chave: Gulf of mexico; Sulfate reducing bacteria; Egyptian continental margin; Northeast pacific ocean; Ribosomal rna sequences; In situ hybridization; Anaerobic oxidation; Marine sediments; Gas hydrate; Oxiding bacteria. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6201.pdf |
| |
|
| |
|
|
| Maffei,Eliane Mariza Dortas; Pompolo,Silvia das Graças; Petitpierre,Eduard. |
| The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In situ hybridization; RDNA sequences; Sequential FISH/Ag-NOR staining; C-banding; Cycloneda sanguinea. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011 |
| |
|
| |
|
| |
|
| |
|
|
| Durand, S; Lightner, Dv; Bonami, Jr. |
| BP-type viruses infect wild and farmed shrimp species in the Americas and can cause high mortality in larval stages. Histologically, BP disease is characterized by the presence of occlusion bodies (OBs) in the nucleus of hepatopancreatic epithelial cells. By in situ hybridization using different molecular probes, we tested shrimp infected with BP-type viruses collected from several geographical areas. In one shrimp, a case of double infection by 2 different BP-type viruses was noted. This has not been previously reported. The molecular probes recognized only the Pacific strain of BP. This specificity of the probe was confirmed by in situ hybridization tests with some infected shrimp collected from the Atlantic and Pacific coasts. The probes reacted only... |
| Tipo: Text |
Palavras-chave: Baculovirus; BP; In situ hybridization; Nonradioactive probes; Shrimp. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00447/55821/62253.pdf |
| |
|
| |
|
|
| Lopes,L.F.; Bacchi,M.M.; Elgui-de-Oliveira,D.; Zanati,S.G.; Alvarenga,M.; Bacchi,C.E.. |
| Epstein-Barr virus (EBV) is a ubiquitous herpesvirus, and most people have serological evidence of previous viral infection at adult age. EBV is associated with infectious mononucleosis and human cancers, including some lymphomas and gastric carcinomas. Although EBV was first reported in lymphoepithelioma-like gastric carcinoma, the virus was also found in conventional adenocarcinomas. In the present study, 53 gastric carcinomas diagnosed in São Paulo State, Brazil, were evaluated for EBV infection by non-isotopic in situ hybridization with a biotinylated probe (Biotin-AGACACCGTCCTCACCACCC GGGACTTGTA) directed to the viral transcript EBER-I, which is actively expressed in EBV latently infected cells. EBV infection was found in 6 of 53 (11.32%) gastric... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epstein-Barr virus; Gastric carcinoma; In situ hybridization; Brazil. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100016 |
| |
|
| |
|
|
| Gagne, N; Cochennec, Nathalie; Stephenson, M; Mcgladdery, S; Meyer, G; Bower, S. |
| As part of a disease resistance experiment, 112 apparently healthy European flat oysters Ostrea edulis L. were exported from Canada (Nova Scotia) into France to test their susceptibility to Bonamia ostreae infection. Twelve oysters died in transit and 17 others died within 2 wk of laboratory quarantine acclimation. All oysters were examined histologically, and the 17 that died during quarantine were assayed for microcells (Bonamia sp. and Mikrocytos mackini) using molecular techniques. A microcell parasite was detected in the connective tissue of 5 of the 112 oysters. Morphological appearance, tissue affinity and molecular characterization through PCR, in situ hybridization (ISH), fluorescence in situ hybridization (FISH) and sequencing revealed a protist... |
| Tipo: Text |
Palavras-chave: Histology; PCR; In situ hybridization; Ostrea edulis; Mikrocytos. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4475.pdf |
| |
|
| |
|
|
| LÓPEZ,MAURICIO; FIGUEROA,JAIME; KAUSEL,GUDRUN; VERA,MARÍA INÉS; KRAUSKOPF,MANUEL. |
| We isolated and cloned a carp somatolactin SL DNA fragment, of which 78% of the nucleotides were identical to the corresponding salmon SL sequence. The results obtained upon Northern blot hybridization of carp pituitary RNA allowed the identification of two transcripts as described for other fish. When the content of SL transcripts in pituitary sections from summer- and winter- acclimatized carp was quantified by in situ hybridization assays, we found no significant differences between the two seasons. In salmonids, plasma SL reaches higher levels in summer than in winter in synchrony with the water temperature cycle; in the eurythermal carp, however, the complex adaptive responses imposed by seasonal environmental changes do not seem to include the... |
| Tipo: Journal article |
Palavras-chave: Acclimatization; In situ hybridization; Somatolactin expression; Pituitary; Carp; Fish. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000100010 |
| |
|
| |
|
|
| Santos,Fernanda Motta da Costa; Torres,Giovana Augusta; Techio,Vânia Helena; Pereira,Antônio Vander; Davide,Lisete Chamma. |
| Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pennisetum glaucum; Pennisetum purpureum; In situ hybridization; Intergenomic pairing; Interspecific hybridization. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814 |
| |
|
|
| Nome,Claudia Fernanda; Nome,Sergio Fernando; Guzmán,Fabiana; Conci,Luis; Laguna,Irma Graciela. |
| Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus (SPMSV). Both diseases cause a synergism between the potyviruses (SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sweet potato chlorotic stunt virus; HSP70h; In situ hybridization; Crinivirus; Sweet potato. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100003 |
| |
|
|
| Gruenke, S.; Lichtschlag, A.; De Beer, D.; Felden, J.; Salman, V.; Ramette, A.; Schulz-vogt, H. N.; Boetius, A.. |
| This study investigated the bacterial diversity associated with microbial mats of polar deep-sea cold seeps. The mats were associated with high upward fluxes of sulfide produced by anaerobic oxidation of methane, and grew at temperatures close to the freezing point of seawater. They ranged from small patches of 0.2-5 m in diameter (gray mats) to extensive fields covering up to 850 m(2) of seafloor (white mats) and were formed by diverse sulfide-oxidizing bacteria differing in color and size. Overall, both the dominant mat-forming thiotrophs as well as the associated bacterial communities inhabiting the mats differed in composition for each mat type as determined by microscopy, 16S rRNA gene sequencing and automated ribosomal intergenic spacer analysis.... |
| Tipo: Text |
Palavras-chave: Mosby mud volcano; Gulf of mexico; Eastern mediterranean sea; Filamentous sulfur bacteria; Sulfide oxidizing bacteria; In situ hybridization; Sulfate reduction; Microbial communities; Marine sediments; Beggiatoa spp. |
| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00212/32360/30823.pdf |
| |
|
|
| Audemard, Corinne; Le Roux, Frédérique; Barnaud, Antoine; Collins, Catherine; Sautour, B; Sauriau, Pierre-guy; De Montaudouin, X; Coustau, C; Combes, C; Berthe, Franck. |
| Marteilia refringens is a major pathogen of the European flat oyster, Ostrea edulis Linnaeus. Since its description, the lifecycle of this protozoan parasite has eluded discovery. Attempts to infect oysters experimentally have been unsuccessful and led to the hypothesis of a complex life-cycle involving several hosts. Knowledge of this life-cycle is of central importance in order to manage oyster disease. However, the exploration of M. refringens life-cycle has been previously limited by the detection tools available and the tremendous number of species to be screened in enzootic areas. In this study, these two restrictions were circumvented by the use of both molecular detection tools and a mesocosm with low biodiversity. Screening of the entire fauna of... |
| Tipo: Text |
Palavras-chave: Experimental transmission; In situ hybridization; PCR; Paracartia grani; Ostrea edulis; Marteilia refringens. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2778.pdf |
| |
| Registros recuperados: 21 | |
|
|
|
